Biotechnology

Biotechnology #1. a) The Use of a Bacterial Plasmid to dead ringer and Sequence a Human Gene The process begins with fix endonucleases scanning and binding to double-stranded deoxyribonucleic acid at specific base-pair sequences, the schooling sites, in a predictable manner. The restriction sites are unremarkably 4 to 8 base pairs long and are characterized by the palindromic sequences, with both strands having the same sequence when read in obstructor direction.

After the restriction endonuclease binds, it starts to disrupt, using hydrolysis, the phosphodiester bonds between neighbor nucleotides, suffice the H-bonds between base pairs in the deoxidiseting region to be broken. This cuts the received double-stranded DNA strand, producing two DNA fragments, which may dissent for polar restriction endonucleases, depending on where the phosphodiester bond is broken when cut by the endonuclease. This process can produce either girdle ends (where ends of the DNA fragment are fully paired with no overhangs),...If you deprivation to get a full essay, order it on our website: BestEssayCheap.com

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